NK Cell Subpopulation Is Altered and the Expression of TLR1 and TLR9 Is Decreased in Patients with Acute Lymphoblastic Leukemia

Figure 1

Characterization of NK cell subpopulations using flow cytometry. (a) First, the NK^total cells [CD14−, CD19−, CD3−, and CD56+], NK^dim [CD56^low CD3−], and NK^bright [CD56^high CD3−] were detected, and subsequently, the subpopulations (I–V) based on the expression of CD56/CD16 were detected. (b) Identification of all NK cells in the peripheral blood of controls and ALL patients, and in the bone marrow of ALL patients. Representative flow cytometry results based on CD56/CD3 expression for each study group are shown. (c) Graphs showing the percentage of NK^total, CD56^bright, and CD56^dim cells (median ± ranges) in the peripheral blood of 10 controls and 9 patients with ALL and in the bone marrow of 10 patients with ALL. . NK, natural killer; ALL, acute lymphoblastic leukemia.

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