miR-1224-3p Promotes Breast Cancer Cell Proliferation and Migration through PGM5-Mediated Aerobic Glycolysis

Figure 2

PGM5 suppresses proliferation and migration of breast cancer cells. (a) Cell proliferation assays of ZR75-1 (left panel) and MCF7 cells (right panel) transfected with the empty vector or PGM5. PGM5 expression was examined by immunoblot with actin as a loading control. (b) Cell proliferation assays of ZR75-1 (left panel) and MCF7 cells (right panel) transfected with control siRNA, PGM5 siRNA, or PGM5 siRNA plus siRNA-resistant PGM5 expression vector (PGM5-R). PGM5 expression was detected by immunoblot. (c, d) Wound healing assays of ZR75-1 and MCF7 cells transfected as in (a) and (b), respectively. The values of the control group in ZR75-1 and MCF7 cells were set to 1. (e, f) Immunoblot analysis of ZR75-1 cells transfected with the empty vector or PGM5 or control siRNA, PGM5 siRNA, or PGM5 siRNA plus PGM5-R with the indicated antibodies. Data shown are mean ± SD of triplicate measurements that were repeated three times with similar results (a–d). ; .

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