miR-1224-3p Promotes Breast Cancer Cell Proliferation and Migration through PGM5-Mediated Aerobic Glycolysis

Figure 3

miR-1224-3p inhibits PGM5 expression by directly targeting its 3’-UTR. (a) ZR75-1 cells were transfected with NC (negative control) or mimics of candidate miRNAs as indicated. PGM5 expression was tested by immunoblot. (b, c) ZR75-1 and MCF7 cells were transfected with (b) NC or miR-1224-3p mimics and (c) scramble control or miR-1224-3p inhibitor (anti-miR-1224-3p). Histograms show relative miR-1224-3p expression by RT-qPCR. (d) RT-qPCR analysis of PGM5 mRNA expression in ZR75-1 and MCF7 cells transfected as in (b) and (c). (e) miRNA luciferase reporter assays in ZR75-1 and MCF7 cells transfected with wild-type (WT) or mutated (MUT) PGM5 reporter plus miR-1224-3p mimics. The top panel indicates WT and MUT forms of putative miR-1224-3p target sequences of PGM5 3’-UTR. Blue font indicates the putative miR-1224-3p binding sites within human PGM5 3’-UTR. Red font indicates the mutations introduced into the PGM5 3’-UTR. Data shown are mean ± SD of triplicate measurements that were repeated three times with similar results. .

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