miR-1224-3p Promotes Breast Cancer Cell Proliferation and Migration through PGM5-Mediated Aerobic Glycolysis

Figure 4

miR-1224-3p promotes cell proliferation and migration through inhibiting PGM5 expression. (a, b) Cell proliferation assays of ZR75-1 cells (left panel) or MCF7 cells (right panel) transfected with (a) miR-1224-3p mimics or miR-1224-3p mimics plus PGM5 expression plasmid or (b) anti-miR-1224-3p, PGM5 siRNA, or anti-miR-1224-3p plus PGM5 siRNA as indicated. Immunoblot analysis shows PGM5 expression. RT-qPCR shows miR-1224-3p expression. (c, d) Wound healing assays of ZR75-1 and MCF7 cells transfected as in (a) and (b). Histograms show relative cell migration. (e, f) Immunoblot analysis of ZR75-1 cells transfected as in (a). Data shown are mean ± SD of triplicate measurements that were repeated three times with similar results (a–d). ; .

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