miR-1224-3p Promotes Breast Cancer Cell Proliferation and Migration through PGM5-Mediated Aerobic Glycolysis

Figure 5

The miR-1224-3p/PGM5 axis modulates glycolysis in breast cancer cells. (a, b) The production of lactate, ATP, and G6P was determined in ZR75-1 and MCF7 cells transfected with (a) empty vector or PGM5 or (b) NC, PGM5 siRNA, or PGM5 siRNA plus PGM5-R. Immunoblot analysis indicates PGM5 expression. (c) The production of lactate, ATP, and G6P was detected in ZR75-1 and MCF7 cells transfected with miR-1224-3p or miR-1224-3p plus PGM5-expressing plasmid. Representative immunoblot reveals PGM5 expression. RT-qPCR indicates miR-1224-3p expression. (d) ZR75-1 and MCF7 cells were transfected as in (a) or (b). Expression of LDHA, LDHB, and PGM5 was detected by immunoblot analysis. (e) Immunoblot analysis of ZR75-1 and MCF7 cells transfected as in (c). Data shown are mean ± SD of quintuplicate measurements that were repeated three times with similar results. Data shown are mean ± SD of triplicate measurements that were repeated three times with similar results (c for RT-qPCR analysis). .

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