miR-1224-3p Promotes Breast Cancer Cell Proliferation and Migration through PGM5-Mediated Aerobic Glycolysis

Figure 6

The miR-1224-3p/PGM5 axis modulates breast cancer cell proliferation and migration through aerobic glycolysis. (a) The proliferation curve of ZR75-1 and MCF7 cells transfected with miR-1224-3p or scramble and treated with 2.5 mM 2-DG as indicated. Representative immunoblot reveals the expression of PGM5. RT-qPCR indicates miR-1224-3p expression. (b) Wound healing assays of ZR75-1 and MCF7 cells transfected as in (a). (c) The proliferation curve of ZR75-1 and MCF7 cells transfected with NC or PGM5 siRNA and treated with 2.5 mM 2-DG as indicated. Representative immunoblot reveals the expression of PGM5. (d) Wound healing assays of ZR75-1 and MCF7 cells transfected as in (c). Histograms show relative cell migration. All values shown are mean ± SD of triplicate measurements and have been repeated 3 times with similar results. ; .

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