Heat Killed Salmonella typhimurium Protects Intestine Against Radiation Injury Through Wnt Signaling Pathway
HKST treatment promoted the activation of DDR pathway, and the stimulated activity was diminished by ICG-001. HIEC cells were pretreated with HKST alone or in combination with ICG-001 for 12 h prior to radiation exposure (8 Gy). Proteins were extracted at 8 hours postradiation, and then the levels of phosphorylated‐ATR (P-ATR), ATR, phosphorylated‐CHK1 (P-CHK1), CHK1, phosphorylated‐ATM (P-ATM), ATM, phosphorylated‐CHK2 (P-CHK2), CHK2, p21, and MyD88 were detected with Western blot (a, b). The levels of P-ATM (c), P-ATR(d), p21(e), P-CHK2 (f), P-CHK1(g), and MyD88 (h) were measured by image J software and analyzed with GraphPad prism 5. .
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