Exploration of the Key Proteins in the Normal-Adenoma-Carcinoma Sequence of Colorectal Cancer Evolution Using In-Depth Quantitative Proteomics

Figure 5

RRP12 and SERPINH1 knockdown reduces viability and proliferation of adenoma organoids, respectively. (a) Representative images of adenoma organoids after 6 and 9 days of culture. (b) The mRNA expression levels of SERPINH1 and RRP12 in SW480 cells were evaluated by qPCR after knockdown mediated by 3 siRNA sequences. (c) SERPINH1 and RRP12 DNA copy number in SW480 cells was evaluated by ddPCR after knockdown mediated by siRNA sequences 2 and 3, respectively. (d) The viability of adenoma organoids significantly decreased after SERPINH1 and RRP12 knockdown compared with the control group. (e) Ki-67 immunostaining showed decreased proliferation of adenoma organoids after SERPINH1 and RRP12 knockdown compared with the control group.

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