POU2F1 Promotes Cell Viability and Tumor Growth in Gastric Cancer through Transcriptional Activation of lncRNA TTC3-AS1

Figure 4

POU2F1 and TTC3-AS1 were upregulated in GC tumor tissues and cell lines. The RNA expression of (a) POU2F1 and (b) lncRNA TTC3-AS1 in GC tumor tissues and normal control tissues was detected using qRT-PCR. (c) POU2F1 protein expression in GC tumor tissues and normal control tissues was detected using western blot. The RNA expression of (d) POU2F1 and (e) lncRNA TTC3-AS1 in GC cell lines of MGC803, BGC823, MKN28, and SGC7901 and the normal gastric epithelial cell line (GES-1) was detected using qRT-PCR. (f) POU2F1 protein expression in GC cell lines of MGC803, BGC823, MKN28, and SGC7901 and GES-1 cells was detected using western blot. All experiments were performed in triplicate. The results were expressed as mean ± SD. , , and versus normal group or GES-1.

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