POU2F1 Promotes Cell Viability and Tumor Growth in Gastric Cancer through Transcriptional Activation of lncRNA TTC3-AS1

Figure 5

lncRNA TTC3-AS1 promoted the cell viability, invasion, and migration of GC cells but had no effect on POU2F1. (a) The subcellular distribution of lncRNA TTC3-AS1 in SGC7901 cells. (b) TTC3-AS1 was, respectively, knocked down by siRNA transfection and overexpressed by plasmid transfection; SGC7901 cells were divided into three groups, NC, TTC3-AS1-KD, and TTAC3-AS1-OE, and function experiments were performed. (c) Cell viability was detected using CCK-8 assays, and cell migration and invasion were, respectively, detected using (d) scratch assay and (e) transwell assay. (f) Protein expression of POU2F1, E-cad, and FN1 was detected using western blot. (g) The effects of TTC3-AS1 knockdown or overexpression on POU2F1 mRNA expression were evaluated using qRT-PCR. All experiments were performed in triplicate. The results were expressed as mean ± SD. , , and versus NC group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)