TUC338 Promotes Diffuse Large B Cell Lymphoma Growth via Regulating EGFR/PI3K/AKT Signaling Pathway

Figure 3

miR-28-5p is sponged by TUC338 in DLBCL cells. (a) qRT-PCR analysis detecting subcellular localization of TUC338 in DLBCL cell lines. (b) Two online tools predicting the miRNAs bound by TUC338. (c) RNA pull-down assay using biotin-labeled probe, followed by qRT-PCR analysis of miRNA enrichment. (d, e) The wild-type or mutant TUC338 sequence was cloned into pmirGLO vector, and luciferase reporter assay was performed in DLBCL cells co-transfected with miR-28-5p mimics and the above pmirGLO vector. (f) qRT-PCR analysis of miR-28-5p expression in paired DLBCL and normal tissues. (g) The correlation analysis between TUC338 and miR-28-5p in DLBCL tissues. (h) qRT-PCR analysis of miR-28-5p expression after TUC338 knockdown. (i) Cell proliferation assay in TUC338-silenced DLBCL cells transfected with miR-28-5p inhibitor. , , .

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