MiR-186 Suppressed Growth, Migration, and Invasion of Lung Adenocarcinoma Cells via Targeting Dicer1

Wang, Juan; Zhang, Yi; Ge, Fanghong

doi:https://doi.org/10.1155/2021/6217469

Journal of Oncology

On this page

Abstract Introduction Materials and Methods Results Discussion Data Availability Ethical Approval Consent Conflicts of Interest Authors’ Contributions References Copyright Related Articles

Special Issue

The Role of Non-Coding RNA in the Regulation of Tumor Signaling Pathways

View this Special Issue

Research Article | Open Access

Volume 2021 | Article ID 6217469 | https://doi.org/10.1155/2021/6217469

MiR-186 Suppressed Growth, Migration, and Invasion of Lung Adenocarcinoma Cells via Targeting Dicer1

Juan Wang,¹Yi Zhang,²and Fanghong Ge³

Academic Editor: Xiangya Ding

Received08 Oct 2021

Revised26 Oct 2021

Accepted29 Oct 2021

Published11 Nov 2021

Abstract

Objective. Globally, the fatal form of lung cancer is non-small-cell lung cancer (NSCLC), and its most common subtype is lung adenocarcinoma (LUAD). In cancer development and progression, miRNAs play key roles primarily in interacting with cancer-related genes. The main focus of this research was to examine the biological roles of miR-186 in LUAD. Methods. We examined tissues of LUAD and lung cancer cell lines. The expressions of miR-186, Dicer1, Ki-67, and PCNA were determined by immunohistochemistry (IHC), real-time quantitative PCR (RT-PCR), and western blot assays. The CCK-8 and transwell assays were used to determine cell proliferation, migration, and invasion. To determine the association between miR-186 and Dicer1, a luciferase assay was used. Results. MiR-186 expression was found to be lower in LUAD tissues, and this was correlated to TNM stage and lymph node metastasis in LUAD patients. miR-186 upregulation significantly reduced the proliferation rate and the level of Ki67 and PCNA of LUAD cell lines HCC827 and A549. Transwell assay exhibited that miR-186 upregulation considerably reduced HCC827 and A549 cells' migration and invasion abilities. Furthermore, we also confirmed that Dicer1 was a direct target of miR-186. Importantly, Dicer1 overexpression abolished the suppression of miR-186 mimics on cell proliferation, migration, and invasion of HCC827 and A549 cells. Conclusion. These results indicated that the miR-186/Dicer1 pathway is critical for regulating LUAD cell proliferation, migration, and invasion.

1. Introduction

Lung cancer is the most common type of cancer in the world, and non-small-cell lung cancer (NSCLC) is the most prevalent subtype of lung cancer (85%) [1, 2]. Although advancements in diagnosing and treating have been made in recent years, patients with NSCLC still have a dismal five-year survival rate [3, 4]. Lung adenocarcinoma (LUAD) is the most prevalent subtype of NSCLC, accounting for 47% of cases. In comparison, the prevalence of two other prominent subtypes of NSCLC, lung squamous cell carcinoma (LUSC) and large-cell carcinoma, is 35% and 17%, respectively [5]. As a result, it is worthwhile to investigate the molecular pathway through which LUAD develops.

Micro RNAs (small noncoding RNAs) inhibit target genes posttranscriptionally. Previous studies have suggested that miRNAs work critically in regulating cancer progression and development via interacting with cancer-related genes. For instance, miR-597-5p inhibited cell proliferation, lowered relative wound width, restricted colony formation, and downregulated invasive cell population in pancreatic cancer and increased cell death via ELK1 targeting [6]. miR-452 was also overexpressed in gastrointestinal cancer tissues and lines of cell and increased tumor cell proliferation, migration, and S-phase arrest by inhibiting EPB41L3. In the study of Liao et al. [7], the miR-206 was reported to decrease the NSCLC cell proliferation, migration, and invasion by downregulating the CORO1C. Hence, miRNA expression and biological activity in cancers must be studied to help find diagnostic and therapeutic targets for tumors.

In the variety of malignancies, miR-186 has been widely investigated, including LUAD, but its biological functions in lung cancer remain contentious [8]. MiR-186 inhibition had previously been revealed to reduce NSCLC cell proliferation, migration, and invasion [9].

Upregulation of miRNA-186 has been reported to reduce cisplatin resistance of NSCLC cells via targeting SIX1. In a previous study, miR-186 had been reported to reduce the inhibited PMI of NSCLC cells [10]. The inhibition of lung cell proliferation is associated with miR-186 targeting SIRT6 [11]. Moreover, miR-186 downregulation is related to poor survival in LUAD as it mediated the inhibition of cell-cycle progression [12]. In contrast, in the research conducted by Feng et al., miR-186 was found capable of increasing LUAD cell growth, migration, and invasion by targeting PTEN [13]. Therefore, we hypothesized that miR-186 may function as a tumor-suppressor in LUAD.

In our current study, we observed that miR-186 levels were lower in LUAD tissues and were linked to lymph node and TNM stage metastases in LUAD patients. Moreover, the upregulation of miR-186 decreased cell proliferation, migration, and invasion through targeting Dicer1.

2. Materials and Methods

2.1. Clinical Data

We collected 35 LUAD tumor tissues and corresponding normal tissues at Cancer Hospital Affiliated with Nantong University between February 2017 and February 2019. All patients with LUAD did not undergo radiotherapy or chemotherapy before surgery. The Ethical Committee of the Cancer Hospital Affiliated with Nantong University approved this research, and all patients involved in the study signed the written consent form.

2.2. Immunohistochemistry (IHC) Assay

For the IHC assay, formalin was used to fix tumor tissues and normal tissues, and then, tissues were embedded in paraffin. The sections were incubated with Dicer1 antibody (Abcam) overnight at 4 °C. Following incubation with a biotinylated secondary antibody, the sections were stained with hematoxylin. The IHC scores were assessed as previously described [14].

2.3. LUAD Cell Lines

Four human cell lines (HCC827 [15], A549 [16], NCI–H23 [17], and NCI–H358 [18]) and the ATCC (Rockville, MD, USA), a standard human lung epithelial cell line BEAS-2B was provided. DMEM, Thermo Fisher, USA, containing 10% fetal bovine serum (FBS, Hyclone, USA) was used to culture A549 and BEAS-2B cells. HCC827, NCI–H23, and NCI–H358 were grown in RPMI-1640 medium containing 10% FBS. All cells were cultured in a humidified atmosphere with 5% CO₂, at room temperature.

2.4. The Extraction of RNA and Real-Time Quantitative PCR (RT-qPCR)

We isolated the total RNA of patient tissue samples or indicated cells by utilizing Trizol reagent (Invitrogen, USA). The PrimeScriptTM RT reagent kit (Takara, Dalian) was used to generate the cDNA (Takara, Dalian), and qPCR was conducted using the SYBR Premix Ex Taq II kit. All specific primers are mentioned as follows:

forward primer (F) 5′-3' and reverse primer (R) 5′-3'. miR-186, F : ACACTCCAGCTGGGCAAAGAATTCTCCTTT, R : CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGAGCCCAAA; Dicer1, F : GAGCTGTCCTATCAGATCAGGG, R : ACTTGTTGAGCAACCTGGTTT; Ki67, F : GGGCCAATCCTGTCGCTTAAT, R : GTTATGCGCTTGCGAACCT; PCNA, F : CCTGCTGGGATATTAGCTCCA, R : CAGCGGTAGGTGTCGAAGC; U6 : F: AAAGCAAATCATCGGACGACC, R : GTACAACACATTGTTTCCTCGGA; and β-actin, F : CTTAGTTGCGTTACACCCTTTCTTG, R : CTGTCACCTTCACCGTTCCAGTTT. U6 or β-actin were considered as endogenous controls.

2.5. Cell Transfection

Random RNAs were designed as control mimics and control inhibitors. The mimics and inhibitors for miR-186/control and the Dicer1 cDNA that was cloned into the pcDNA3.1 vector (pcDNA-Dicer1) were designed and manufactured by GenePharma (Shanghai, China). The pcDNA3.1 empty vector was used as a negative control (pcDNA) (GenePharma, Shanghai, China). Lipofectamine 2000 (Invitrogen) was used to transfect the cells.

2.6. CCK-8 Assay

Cells (1000 cells per well) were transfected with miR-186 mimics, or pcDNA-Dicer 1 was grown in 96-well plates. After 0, 24, 48, and 72 h, each well was treated with 10 μl solution of CCK-8, and the cells were incubated at 37 °C for 4 hours. The plate reader was used to measure absorbance at 450 nm.

2.7. Migration and Invasion Assay

A BD BioCoat Chamber (BD Biosciences) was utilized to observe cell migration and invasion activity. Cells (5 × 10⁴/ml) transfected with miR-186 mimics or pcDNA-Dicer 1 in the growth medium without serum and were seeded into the upper chamber with or without a precoating Matrigel matrix. The lower wells were filled with the complete medium. The penetrated cells were fixed, stained with crystal violet, and counted under microscopy after 24 hours.

2.8. Luciferase Assay

The psi-CHECK-2 luciferase reporter vectors (LRVs) with the wild-type (WT) or mutant Dicer 1–3′UTR were generated by GenePharma. Cells were cotransfected with LRV and miR-186 mimics or negative control miRNA. After 24 h of transfection, the dual-luciferase assay kit (Promega) was used to assess luciferase activity.

2.9. Western Blot

Total protein was extracted from transfected cells using ProteoJET Mammalian Cell Lysis Reagent (MBI Fermentas) containing a proteinase inhibitor cocktail (Boster, China). Equal amounts of proteins were separated using SDS-PAGE. At 4°C overnight, the membranes were incubated with primary antibodies. Super ECL Plus Detection Reagent (Applygen Technologies Inc., China) was applied for detecting the protein signal. Dicer 1 (Abcam, ab259327, 1 : 1000) and GAPDH (Abcam, ab9485, 1 : 1000) were used as primary antibodies.

2.10. Statistical Analysis

All data were presented as the mean ± standard error (SD) and analyzed using Student's t-test or one-way ANOVA test between two or more groups. , , and were considered statistically significant.

3. Results

3.1. Expressions of miR-186 in LUAD Tissue and Cell Lines

To demonstrate the function of miR-186 and its overexpression in the pathogenesis of LUAD, RT-qPCR was performed. Data showed a lower miR-186 expressions in LUAD tissues than normal tissues (Figure 1(a)). Furthermore, the negative correlation of the miR-186 expressions in LUAD tissues with TNM stage and lymph node metastasis was observed (Figures 1(b) and 11(c)). Additionally, in HCC827, A549, NCI–H23, and NCI–H358, the level of miR-186 was lower than that in a standard human lung epithelial cell line BEAS-2B (Figure 1(d)).

(a)

(b)

(c)

(d)

Figure 1

Decreased levels of miR-186 expressions in LUAD tissues and cell lines. (a) Normal tissues (n = 35) and LUAD tissues (n = 35), RT-qPCR analysis was performed to evaluate the expressions of mRNA. (b) mRNA expression of miR-186 was associated with the TNM stage of LUAD patients. (c) mRNA expression was related with lymph node metastasis. (d) mRNA expressions of miR-186 in the normal human lung epithelial cell line BEAS-2B and LUAD cell lines (HCC827, A549, NCI–H23, and NCI–H358). , , and .

3.2. Effect of miR-186 on Cell Proliferation

In HCC827 and A549 cells, miR-186 mimics treatment significantly upregulated the miR-186 levels (Figure 2(a)). The results of the CCK-8 assay showed that overexpression of miR-186 significantly decreased the proliferation rate of HCC827 and A549 cells (Figure 2(b)). Moreover, the upregulation of miR-186 markedly reduced the levels of Ki67 and PCNA, two cell proliferation markers (Figure 2(c)). These results verify that the upregulation of miR-186 levels was able to inhibit the cell proliferation of LUAD.

(a)

(b)

(c)

3.3. Effect of miR-186 on Cell Migration and Invasion

The function of miR-186 in regulating the cell migration and invasion potential of LUAD cells was accessed by using transwell assay. MiR-186 upregulation significantly reduced the migration and invasion abilities of HCC827 and A549 cells when compared to the control group, as shown in Figures 3(a) and 3(b).

(a)

(b)

3.4. Identification of Dicer1 as a Target of miR-186

TargetScan (http://www.targetscan.org), a bioinformatics algorithm, was used to predict Dicer1 as a possible target of miR-186 (Figure 4(a)). The luciferase reporter assay demonstrated that the upregulation of miR-186 considerably decreased the luciferase activity of luciferase reporter containing WT-Dicer1-3′UTR in HCC827 and A549 cells (Figure 4(b)), whereas miR-186 upregulation did not influence the luciferase activity of luciferase reporter containing mutant-Dicer1-3′UTR in HCC827 and A549 cells (Figure 4(b)). Moreover, miR-186 upregulation substantially reduced the protein and mRNA expressions of Dicer1, while a considerable increase was seen in the protein and mRNA expressions of Dicer1 by miR-186 downregulation (Figures 4(c) and 4(d)).

(a)

(b)

(c)

(d)

3.5. Dicer1 Was Correlated Negatively with miR-186 in LUAD Tissue

The Dicer1 protein expression level was much elevated in LUAD tissues than in normal (Figure 5(a)). Additionally, the degree of Dicer1 expression in LUAD tissues was notably corrected with the TNM stage and lymph node metastasis (Figures 5(b) and 5(c)). Importantly, the protein expression level of Dicer1 in the miR-186 low expression group was higher than that in the miR-186 low expression group (Figure 5(d)).

(a)

(b)

(c)

(d)

3.6. miR-186 Inhibited Cell Proliferation, Migration, and Invasion via Dicer1

HCC827 and A549 cells were cotransfected with miR-186 mimics and pcDNA-Dicer1. The transfection with miR-186 mimics induced the growth suppression of HCC827 and A549 cells, while cotransfection with miR-186 mimics and pcDNA-Dicer1 rescued the suppression of miR-186 on cell proliferation (Figure 6(a)). Furthermore, cotransfection of HCC827 and A549 cells with miR-186 mimics and pcDNA-Dicer1 removed the inhibition of miR-186 on migratory and invasion capabilities (Figure 6(b)).

(a)

(b)

4. Discussion

Studies have revealed that miRNAs have been associated with tumor progression [19,20]. In oral squamous cell carcinoma, miR-146b inhibition suppressed migration, cell proliferation, and invasion via binding and downregulating HBP1 expression [10] and in the colorectal cancer miR-576-5p-targeted Wnt5a-mediated Wnt/β-catenin signaling pathway by inducing epithelial-to-mesenchymal transition [21]. Through targeting TrxR2 in lung adenocarcinoma cells, the miR-195-5p upregulation significantly decreased cell proliferation, migration, and invasion while increasing apoptosis [22]. A recent publication suggested that some miR displays subtype specificity in lung cancer [23]. In our study, the LUAD tissue showed a reduction in miR-186 regulation and cell proliferation migration, and invasion was suppressed by enhanced expressions of miR-186. Further investigation of the regulation and functionality of miR-186 in different subtypes of lung cancer is suggested.

In addition, miR-186 has been shown to effect many biological mechanisms in human cancer [8]. miR-186 modulated the ovarian cancer cells’ cisplatin sensitivity by downregulating PIK3R3 and PTEN while enhancing the regulation of APAF1 [24]. In breast cancer cells, through modulating EREG signaling, miR-186-3p induced aerobic glycolysis and tamoxifen resistance [25]. MiR-186, which uses CDK6 as a direct target, has been shown to decrease in vivo tumor development, cell proliferation, migration, and invasion in renal cell carcinoma. In the G0/G1 phase, it also causes the inhibition of cell-cycle progression and induction of apoptosis [26]. miR-186-5p overexpression can suppress the colorectal cancer cell proliferation, metastasis, and epithelial-to-mesenchymal transition by targeting ZEB1 [27]. In this study, in comparison to the normal tissues, the LUAD tissues were observed with low expression of miR-186. Moreover, the expressions of miR-186 had a negative correlation with the TNM stage and lymph node metastasis. Additionally, miR-186 decreased cell proliferation, migration, and invasion by inhibiting Dicer. Our research has indicated that, in LUAD, miR-186 might have a tumor-suppressor function.

Dicer1 is a well-conserved RNaseIII endoribonuclease that contributes to microRNA biogenesis (miRNAs) [28]. Previous studies indicated that Dicer1 has a critical part in tumorigenesis and progression [29]. In colon cancer, Dicer1 impairment promoted stemness, induced an epithelial-to-mesenchymal transition, and enhanced metastatic capability of cancerous colon cells [30]. It is reported that Dicer1 was involved in miR-191/425-mediated promotion of breast cancer proliferation and metastasis [31]. The study by Ramírez-Moya et al. showed that a positive feedback loop between Dicer1 and differentiation transcription factors is essential for tumorigenesis in thyroid cancer [32]. We found that Dicer1 was an miR-186's target. miR-186 can directly reduce Dicer1 expression directly in A549, and HCC827 cells are demonstrated by the luciferase assay, RT-qPCR, and western blot assay. Moreover, Dicer1 expression levels were correlated negatively with miR-186 levels in LUAD tissue. Additionally, Dicer1 overexpression abolished the suppression of miR-186 on cell proliferation, migration, and invasion of HCC827 and A549 cells.

In summary, our data demonstrated that, in LUAD tissues, miR-186 was decreased, and its correlation with TNM stage and lymph node metastasis was found in LUAD patients. Also, cell proliferation, migration, and invasion were inhibited by the miR-186 overexpression via inhibiting Dicer 1. These findings might serve as the way for the development of novel LUAD-targeted therapies and to design novel miRNA-based therapeutic strategies against LUAD.

Data Availability

Data will be made available on reasonable request.

Ethical Approval

The study was approved by the Ethics Committee of Cancer Hospital Affiliated to Nantong University.

Informed consent was obtained from all individual participants included in the study.

Conflicts of Interest

The authors report no conflicts of interest.

Authors’ Contributions

All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by Fanghong Ge. The first draft of the manuscript was written by Juan Wang and Yi Zhang, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript. Juan Wang and Yi Zhang contributed equally to this work.

References

H. A. Hamann, E. S. Ver Hoeve, L. Carter-Harris, J. L. Studts, and J. S. Ostroff, “Multilevel opportunities to address lung cancer stigma across the cancer control continuum,” Journal of Thoracic Oncology, vol. 13, no. 8, pp. 1062–1075, 2018.
View at: Publisher Site | Google Scholar
P. J. Kuehl, C. M. Yingling, D. Dubose et al., “Inhalation delivery dramatically improves the efficacy of topotecan for the treatment of local and distant lung cancer,” Drug Delivery, vol. 28, no. 1, pp. 767–775, 2021.
View at: Publisher Site | Google Scholar
C. Huang, C. Hung-Che, C. Chun-Ku et al., “Significance of preoperative biopsy in radiological solid-dominant clinical stage i non-small-cell lung cancer,” Interactive Cardiovascular and Thoracic Surgery, vol. 32, no. 4, pp. 537–545, 2020.
View at: Publisher Site | Google Scholar
Y. Wang, M. Shi, N. Yang, X. Zhou, and L. Xu, “GPR115 contributes to lung adenocarcinoma metastasis associated with LAMC2 and predicts a poor prognosis,” Frontiers in oncology, vol. 10, Article ID 577530, 2020.
View at: Publisher Site | Google Scholar
R. Ruiz-Cordero and W. P. Devine, “Targeted therapy and checkpoint immunotherapy in lung cancer,” Surgical Pathology Clinics, vol. 13, no. 1, pp. 17–33, 2020.
View at: Publisher Site | Google Scholar
S. Li, X. Li, X. Xing, and L. Wang, “miR-597-5p inhibits cell growth and promotes cell apoptosis by targeting ELK1 in pancreatic cancer,” Human Cell, vol. 33, no. 4, pp. 1165–1175, 2020.
View at: Publisher Site | Google Scholar
M. Liao and L. Peng, “MiR-206 may suppress non-small lung cancer metastasis by targeting CORO1C,” Cellular and Molecular Biology Letters, vol. 25, no. 1, p. 22, 2020.
View at: Publisher Site | Google Scholar
Z. Wang, H.-H. Sha, and H.-J. Li, “Functions and mechanisms of miR-186 in human cancer,” Biomedicine & Pharmacotherapy, vol. 119, Article ID 109428, 2019.
View at: Publisher Site | Google Scholar
T. Huang, G. Wang, L. Yang et al., “MiR-186 inhibits proliferation, migration, and invasion of non-small cell lung cancer cells by downregulating Yin Yang 1,” Cancer Biomarkers, vol. 21, no. 1, pp. 221–228, 2017.
View at: Publisher Site | Google Scholar
X. Liu, X Zhou, Y Chen, Y Huang, J He, and H Luo, “miR-186-5p targeting SIX1 inhibits cisplatin resistance in non-small-cell lung cancer cells (NSCLCs),” Neoplasma, vol. 67, no. 1, pp. 147–157, 2020.
View at: Publisher Site | Google Scholar
L. Ruan, J. Chen, L. Ruan, T. Yang, and P. Wang, “MicroRNA-186 suppresses lung cancer progression by targeting SIRT6,” Cancer Biomarkers, vol. 21, no. 2, pp. 415–423, 2018.
View at: Publisher Site | Google Scholar
J. Cai, J. Wu, H. Zhang et al., “miR-186 downregulation correlates with poor survival in lung adenocarcinoma, where it interferes with cell-cycle regulation,” Cancer Research, vol. 73, no. 2, pp. 756–766, 2013.
View at: Publisher Site | Google Scholar
H. Feng, Z. Zhang, X. Qing, S. W. French, and D. Liu, “miR-186-5p promotes cell growth, migration and invasion of lung adenocarcinoma by targeting PTEN,” Experimental and Molecular Pathology, vol. 108, pp. 105–113, 2019.
View at: Publisher Site | Google Scholar
Y. Ma, S. Zhan, H. Lu et al., “B7-H3 regulates KIF15-activated ERK1/2 pathway and contributes to radioresistance in colorectal cancer,” Cell Death & Disease, vol. 11, no. 10, Article ID 824, 2020.
View at: Publisher Site | Google Scholar
X.-X. Liu, Y.-E. Yang, X. Liu et al., “A two-circular RNA signature as a noninvasive diagnostic biomarker for lung adenocarcinoma,” Journal of Translational Medicine, vol. 17, no. 1, p. 50, 2019.
View at: Publisher Site | Google Scholar
H. Ashinuma, Y Takiguchi, S Kitazono et al., “Antiproliferative action of metformin in human lung cancer cell lines,” Oncology Reports, vol. 28, no. 1, pp. 8–14, 2012.
View at: Publisher Site | Google Scholar
M. I. Saad, L. McLeod, L. Yu et al., “The ADAM17 protease promotes tobacco smoke carcinogen-induced lung tumorigenesis,” Carcinogenesis, vol. 41, no. 4, pp. 527–538, 2020.
View at: Publisher Site | Google Scholar
S. H. Kim, J. E. Lee, S.-H. Yang, and S. W. Lee, “Induction of cytokines and growth factors by rapamycin in the microenvironment of brain metastases of lung cancer,” Oncology Letters, vol. 5, no. 3, pp. 953–958, 2013.
View at: Publisher Site | Google Scholar
X. Lai, M. Eberhardt, U. Schmitz, and J. Vera, “Systems biology-based investigation of cooperating microRNAs as monotherapy or adjuvant therapy in cancer,” Nucleic Acids Research, vol. 47, no. 15, pp. 7753–7766, 2019.
View at: Publisher Site | Google Scholar
G. H. Smith, P. J. Doherty, R. B. Stead, C. M. Gorman, D. E. Graham, and B. H. Howard, “Detection of transcription and translation in situ with biotinylated molecular probes in cells transfected with recombinant DNA plasmids,” Analytical Biochemistry, vol. 156, no. 1, pp. 17–24, 1986.
View at: Publisher Site | Google Scholar
J. Luo, L. Luying, S. Jinwen et al., “miR-576-5p promotes epithelial-to-mesenchymal transition in colorectal cancer by targeting the Wnt5a-mediated Wnt/β-catenin signaling pathway,” Molecular Medicine Reports, vol. 23, no. 2, 2021.
View at: Publisher Site | Google Scholar
L. Bu, T. Yingxuan, W. Hongqing, J. Weihong, and Y. Shuanying, “miR-195-5p exerts tumor-suppressive functions in human lung cancer cells through targeting TrxR2,” Acta Biochimica et Biophysica Sinica, vol. 53, no. 2, pp. 189–200, 2020.
View at: Publisher Site | Google Scholar
M. L. Davenport, B. E. John, D. S. Austin et al., “miR-31 displays subtype specificity in lung cancer,” Cancer Research, vol. 81, no. 8, pp. 1942–1953, 2021.
View at: Publisher Site | Google Scholar
K. Li, Z. Zheng, L. Ju, and X. Rui, “miR-146b functions as an oncogene in oral squamous cell carcinoma by targeting HBP1,” Technology in Cancer Research and Treatment, vol. 19, no. 1, Article ID 1533033820959404, 2020.
View at: Publisher Site | Google Scholar
M. He, Q. Jin, C. Chen et al., “The miR-186-3p/EREG axis orchestrates tamoxifen resistance and aerobic glycolysis in breast cancer cells,” Oncogene, vol. 38, no. 28, pp. 5551–5565, 2019.
View at: Publisher Site | Google Scholar
Z. Guo, X. Lv, and H. Jia, “MiR-186 represses progression of renal cell cancer by directly targeting CDK6,” Human Cell, vol. 33, no. 3, pp. 759–767, 2020.
View at: Publisher Site | Google Scholar
J. Li, L. Xia, Z. Zhou et al., “MiR-186-5p upregulation inhibits proliferation, metastasis and epithelial-to-mesenchymal transition of colorectal cancer cell by targeting ZEB1,” Archives of Biochemistry and Biophysics, vol. 640, pp. 53–60, 2018.
View at: Publisher Site | Google Scholar
M. Thunders and B. Delahunt, “DICER1:Gene of the month: ruler and controller,” Journal of Clinical Pathology, vol. 74, no. 2, pp. 69–72, 2020.
View at: Publisher Site | Google Scholar
Z. Dobrijević, S Matijašević, T Išić Denčić, D Savić-Pavićević, O Nedić, and G Brajušković, “Association between genetic variants in DICER1 and cancer risk: an updated meta-analysis,” Gene, vol. 766, Article ID 145132, 2021.
View at: Publisher Site | Google Scholar
M. S. Iliou, V. da Silva-Diz, F. J. Carmona et al., “Impaired DICER1 function promotes stemness and metastasis in colon cancer,” Oncogene, vol. 33, no. 30, pp. 4003–4015, 2014.
View at: Publisher Site | Google Scholar
X. Zhang, M. Wu, Q.-Y. Chong et al., “Amplification of hsa-miR-191/425 locus promotes breast cancer proliferation and metastasis by targeting DICER1,” Carcinogenesis, vol. 39, no. 12, pp. 1506–1516, 2018.
View at: Publisher Site | Google Scholar
J. Ramírez-Moya and P. Santisteban, “A positive feedback loop between DICER1 and differentiation transcription factors is important for thyroid tumorigenesis,” Thyroid: Official Journal of the American Thyroid Association, vol. 31, no. 6, pp. 912–921, 2020.
View at: Publisher Site | Google Scholar

Copyright

Copyright © 2021 Juan Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PDF Download Citation

Download other formats

Order printed copies

Views

408

Downloads

538

Citations