MCT4 Promotes Tumor Malignancy in F98 Glioma Cells

Figure 2

MCT4 overexpression and knockdown phenotype in F98 glioma cells. (a) Immunocytological staining for MCT4 expression (red) in GFP-positive con, MCT4, and MCT4^KD F98 cells (green). Scale bar represents 10 μm. Quantification of mean fluorescence intensity (MFI) of the red channel is shown below. Statistical analysis was performed by one-way ANOVA with Tukey’s posttest (; , mean ± SEM, n = 11). (b) MCT4 mRNA expression ratios in con, MCT4, and MCT4^KD F98 cells, normalized to con, as determined by qRT-PCR. Statistical analysis was performed by one-way ANOVA with Tukey’s posttest (^ns; , mean ± SEM, n = 5). (c) MCT4 protein expression in con, MCT4, and MCT4^KD F98 cells determined by flow cytometry analysis. Statistical analysis was performed by one-way ANOVA with Tukey’s posttest (, mean ± SEM, n = 3). (d) Supernatants of con, MCT4, and MCT4^KD F98 cells cultured for 72 h. (e) pH_e measured within the supernatants of con, MCT4, and MCT4^KD F98 cells as well as MCT4 cells treated with 100 μM pCMBS or 150 μM Phl and cultured for 48 h and 72 h. Statistical analysis was performed by two-way ANOVA with Bonferroni posttest (, , and , mean ± SEM, n = 5). (f) Extracellular lactate concentrations measured in the supernatants of con, MCT4, and MCT4^KD F98 cells as well as MCT4 cells treated with 100 μM pCMBS or 150 μM Phl and cultured for 48 h and 72 h. Statistical analysis was performed by two-way ANOVA with Bonferroni posttest (, mean ± SEM, n = 3).

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