MCT4 Promotes Tumor Malignancy in F98 Glioma Cells

Figure 4

Angiogenesis in MCT4 overexpression and knockdown F98 glioma cells. (a) Tube formation of HUVEC in 3D cell culture treated with the conditioned media of con, MCT4, and MCT4^KD F98 cells as well as with the conditioned media of MCT4 cells treated with 100 μM pCMBS, 150 μM Phl, or 10 mM Lac. Images on the left depict raw data, whereas images on the right are postprocessed with Fiji. Scale bar represents 200 μm. Quantification of tube formation parameters for the assessment of angiogenesis was performed with Fiji. Data were normalized to con. Statistical analysis was performed by two-way ANOVA with Bonferroni posttest (; , mean ± SEM, n = 3). (b) VEGF and VEGFR mRNA expression ratios in HUVEC treated with the conditioned media of con, MCT4, and MCT4^KD F98 cells as well as with the conditioned media of MCT4 cells treated with 100 μM pCMBS, 150 μM Phl, or 10 mM Lac, as determined by qRT-PCR. Data were normalized to con. Statistical analysis was performed by one-way ANOVA with Tukey’s posttest (; mean ± SEM, n = 3). (c) Scheme of the procedure for VOGIM slice culture. (d) Blood vessel sprouting in VOGIM slices 5 d after tumor implantation with con, MCT4, and MCT4^KD F98 cells (green) as well as MCT4 cells treated with 100 μM pCMBS or 150 μM Phl (green), visualized by antilaminin staining (red). Quantification of blood vessel sprouting parameters for the assessment of angiogenesis was performed with Fiji. Data were normalized to con. Statistical analysis was performed by two-way ANOVA with Bonferroni posttest (, mean ± SEM, n = 9).

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