In Hepatocellular Carcinoma, miRNA-296-3p Targets MSL2 and Suppresses Cell Proliferation and Invasion

Figure 4

MSL2 is a target gene of miRNA-296-3p. (a) Bioinformatic analysis predicted complementary sequences between miRNA-296-3p and MSL2. The schematic diagram shows the putative binding sites of miRNA-296-3p on the 3′′-UTR region of MSL2. (b) Luciferase reporter assay suggested that miRNA-296-3p mimic reduced the luciferase activity of MSL2 WT in HEK-293T cells. WT: wild type; Mut: mutation. vs. NC mimics, n = 3 per group. (c) mRNA and (d) protein levels of MSL2 in Huh-7 and HepG2 cells transfected with NC mimic or miRNA-296-3p mimic. Values were mean ± SE, vs. control, n = 3 per group.

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