LncRNA LINC01303 Promotes the Progression of Oral Squamous Cell Carcinomas via the miR-429/ZEB1/EMT Axis

Figure 4

LINC01303 directly targeted miR‐429. (a) starBase prediction of LINC01303 and miR-429 sites. Dual luciferase reporter analysis of the targeted relationship between LINC01303 and miR-429 in TSCCA cells (b) and SCC-25 cells (c). qRT‐PCR was used to examine the expression level of miR‐429 after transfection with si‐NC, si‐LINC01303, vector-NC, or vector-LINC01303 in TSCCA cells (d) and SCC-25 cells (e). (f) The linear correlations of LINC01303 and miR-429 expression were demonstrated by Pearson’s analysis. (g) FISH analysis was used to detect the colocalization of LINC01303 and miR-429 in TSCCA cells. The nuclei were stained with DAPI (blue), LINC01303 was stained with Cys (red), and miR-429 was stained with FAM (green). The red arrow shows colocalization of LINC01303 and miR-429. Scale bar: 10 μm. .

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