Metabotropic Glutamate Receptor 8 Is Regulated by miR-33a-5p and Functions as an Oncogene in Breast Cancer

Figure 2

Evaluation GRM8 effects on breast cancer cell growth and apoptosis. (a, b) The expression of GRM8 protein was detected by western blotting assay after SK-BR-3 cells were transected with sh-GRM8/sh-NC or HCC1937 cells were transfected with vector-GRM8/vector-NC. (c, d) CCK-8 assay was used to assess cell growth after SK-BR-3 cells were transected with sh-GRM8/sh-NC or HCC1937 cells were transfected with vector-GRM8/vector-NC. (e, f) Cell apoptosis was detected by flow cytometry assay after SK-BR-3 cells were transected with sh-GRM8/sh-NC or HCC1937 cells were transfected with vector-GRM8/vector-NC. (g, h) CCK-8 and flow cytometry assays were used to detect cell growth and apoptosis in GRM8 overexpressed Hs 578Bst cells .

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