circITGA7 Acts as a miR-370-3p Sponge to Suppress the Proliferation of Prostate Cancer

Figure 2

The enforced expression of circITGA7 inhibits cell proliferation in vitro and in vivo. (a) The expression levels of circITGA7 in PCa cells stably transfected with circITGA7 overexpression vector or negative control vector were detected by qRT-PCR. (b) CCK-8 assay was used to detect the growth rate of transfected PCa cells. (c, d) The cell proliferation was measured by the colony formation and EdU assays. Scale bar, 100 μm. (e) The cell cycle was analyzed by flow cytometry analysis. (f) The expression of circITGA7 in xenograft tumors was detected. (g–i) Xenograft tumors derived from circITGA7 group showed a smaller volume and lower weight than those derived from empty vector group. Data were presented as mean ± SD of three independent experiments. Statistically significant differences were indicated: , .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)