Long Noncoding RNA MALAT1 Interacts with miR-124-3p to Modulate Osteosarcoma Progression by Targeting SphK1

Figure 3

MALAT1 is associated with microRNA-124-3p (miR-124-3p) expression in OS tissues. (a) An inverse correlation between MALAT1 and miR-124-3p expression was observed in OS tissues. (b) Real-time PCR assay showed that knockdown of MALAT1 (si-MALAT1) caused upregulation of miR-124-3p in the MG63 and U2OS cell lines. (c) MALAT1 expression was decreased in response to miR-124-3p overexpression, compared with the miR-NC (NC) group. (d) Generation of MALAT1-WT and MALAT1-MUT containing luciferase reporter vectors by sequentially mutating the predicted miR-124-3p binding site in the MALAT1 3′-untranslated region (3′-UTR). (e) The MALAT1-WT/MALAT1-MUT vectors and miR-NC/miR-124-3p mimics were cotransfected into human embryonic kidney (HEK) 293T cells, respectively. miR-124-3p mimic significantly decreased the luciferase activities of MALAT1-WT compared with mimic-NC. Cotransfection with miR-124-3p mimic and MALAT1-MUT did not alter luciferase activities either. (f, g) MG63 and U2OS cells were transfected with NC, miR-124-3p, miR-124-3p + NC, or miR-124-3p + MALAT1. Cell viability was determined by CCK-8 assay in transfected MG63 and U2OS cells at 24, 48, 72, and 96 h. Results showed that MALAT1 overturns the miR-124-3p induced inhibitory effect on proliferation of OS cells. Data are presented as mean ± SD of three independent experiments. and .

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