Long Noncoding RNA MALAT1 Interacts with miR-124-3p to Modulate Osteosarcoma Progression by Targeting SphK1

Figure 4

Identifying the regulatory relationship between MALAT1, miR-124-3p, and SphK1. (a) The putative binding sequences of miR-124-3p and 3′-UTR of SPHK1. (b) The SphK1-WT/SphK1-MUT vectors and miR-NC/miR-124-3p mimics were cotransfected into 293T cells, respectively. miR-124-3p mimic significantly decreased the luciferase activities of SphK1-WT compared with mimic-NC. Cotransfection with miR-124-3p mimic and SphK1-MUT did not alter luciferase activities either. (c, d) Western blot assay showed that the expression of SphK1 was downregulated by knockdown of MALAT1 and miR-124-3p overexpression in both MG63 and U2OS cells. (e, f) Real-time PCR assay also showed similar results in both MG63 and U2OS cell lines. (g) SphK1 knockdown was achieved by si-SphK1 as demonstrated by Western blot assay. (h, i) CCK-8 assay results showed that SphK1 inhibition by si-SphK1 reduced the proliferation of OS cells. Data are presented as mean ± SD of three independent experiments. and .

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