Circ-DONSON Knockdown Inhibits Cell Proliferation and Radioresistance of Breast Cancer Cells via Regulating SOX4

Figure 2

Circ-DONSON knockdown inhibited BC cell proliferation, reduced the radioresistance of BC cells, and inhibited SOX4 expression. (a) The expression of circ-DONSON in MCF-7 and MDA-MB-231 cells after treatment with circ-DONSON siRNAs. (b) CCK-8 assay was performed on MCF-7 and MDA-MB-231 cells after transfection with circ-DONSON siRNAs. (c) BrdU-ELISA assay was conducted on MCF-7 and MDA-MB-231 cells after transfection with circ-DONSON siRNAs. (d) Cell survival assay was performed on MCF-7 and MDA-MB-231 cells after exposure to 0, 2, 4, 6, or 8 Gy X-ray irradiation and transfection of circ-DONSON siRNAs. (e) Cell viability of MCF-7 and MDA-MB-231 cells after exposure to 4 Gy X-ray irradiation and transfection of circ-DONSON siRNAs was analyzed by CCK-8 assay. (f) The relative caspase-3 viability of MCF-7 and MDA-MB-231 cells after exposure to 4 Gy X-ray irradiation and treatment of circ-DONSON siRNAs. (g) The protein levels of SOX4 in MCF-7 and MDA-MB-231 cells after treatment with circ-DONSON siRNAs. The density of western blots bands was analyzed by ImageJ software. , , and .