Circ-DONSON Knockdown Inhibits Cell Proliferation and Radioresistance of Breast Cancer Cells via Regulating SOX4

Figure 3

Circ-DONSON knockdown inhibited BC cell proliferation and reduced the radioresistance of BC cells via SOX4. (a) The protein levels of SOX4 in MCF-7 and MDA-MB-231 cells after treatment with pcDNA3.1-SOX4. The density of western blots bands was analyzed by ImageJ software. (b) CCK-8 assay was conducted on MCF-7 and MDA-MB-231 cells after cotreatment with circ-DONSON siRNA-1 and pcDNA3.1-SOX4. (c) BrdU-ELISA assay was performed on MCF-7 and MDA-MB-231 cells after cotreatment with circ-DONSON siRNA-1 and pcDNA3.1-SOX4. (d) Cell viability of MCF-7 and MDA-MB-231 cells with cotransfection of circ-DONSON siRNA-1 and pcDNA3.1-SOX4 after exposure to 4 Gy X-ray irradiation was analyzed by CCK-8 assay. (e) Relative caspase-3 viability of MCF-7 and MDA-MB-231 cells with cotreatment of circ-DONSON siRNA-1 and pcDNA3.1-SOX4 after exposure to 4 Gy X-ray irradiation. vs. si-NC and vs. si-circ-DONSON-1+vector.