circSLC30A7 Inhibits Hepatocellular Carcinoma Cell Proliferation via the miR-767-5p/FBXW7/NOTCH1 Axis

Figure 4

circSLC30A7 inhibits HCC cell proliferation and colony formation and accelerates apoptosis by competitively binding to miR-767-5p. Huh-7 cells were transfected with oe-circSLC30A7 (with empty vector as control) or miR-767-5p mimic + oe-circSLC30A7 (with oe-circSLC30A7 + miR-NC as control). HepG2 cells were transfected with sh-circSLC30A7 (with sh-control as control) or sh-circSLC30A7 + anti-miR-767-5p (with sh-circSLC30A7 +  anti-miR-NC as control). (a) RT-qPCR quantitation of miR-767-5p expression in Huh-7 and HepG2 cells. (b) Proliferation of Huh-7 and HepG2 cells determined by CCK-8 method. (c) Colony formation ability of Huh-7 and HepG2 cells evaluated by colony formation assay. (d) Proliferation of Huh-7 and HepG2 cells determined by EdU incorporation assay. (e) Apoptosis of Huh-7 and HepG2 cells measured by flow cytometry. , , . A: empty vector; B: oe-circSLC30A; C: oe-circSLC30A7 + miR--NC; D: oe-circSLC30A7 + miR767-5p-mimic; E: sh-control; F: sh-circSLC30A7; G: sh-circSLC30A7 + anti-miR-NC; H: sh-circSLC30A7 + anti-miR-767-5p.

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