BCL-2 (-938C>A), BAX (-248G>A), and HER2 Ile655Val Polymorphisms and Breast Cancer Risk in Indian Population

Table 1

PCR primers, PCR product sizes, restriction enzymes, and enzyme digests of BCL-2 (-938C>A), BAX (-248G>A), and HER2 Ile655Val genes.

Gene	Position and base change	Genotyping	Primer	PCR product	Restriction enzyme used	Enzyme digests
BCL-2	-938C>A	PCR-RFLP	5′-CTGCCTTCATTTAT; CCAGCA-3′ (forward); 5′-GGCGGCAGATGA; ATTACAA-3′ (reverse)	300 bp	BccI (1 unit)	C allele: 189 and 111 bp; A allele: 300 bp
BAX	-248G>A	PCR-RFLP	5′-CATTAGAGCTGCGA; TTGGACCG-3′ (forward); 5′-GCTCCCTCGGGAG; GTTTGGT-3′ (reverse)	109 bp	MspI (1 unit)	G allele: 89 and 20 bp; A allele: 109 bp
HER2	I655V A>G	PCR-RFLP	5′-AGAGCGCCAGCCCTCT; GACGTCCAT-3′ (forward); 5′-TCCGTTTCCTGCAGCA; GTCTCCGCA-3′ (reverse)	148 bp	BsmAI (1 unit)	G allele: 116 and 32 bp; A allele: 148 bp