Nrf3 Promotes 5-FU Resistance in Colorectal Cancer Cells via the NF-κB/BCL-2 Signaling Pathway In Vitro and In Vivo

Figure 2

Nrf3 inhibits the antitumor effect of 5-FU in colon cancer cells. HT29, HT29/Nrf3, SW620, and SW620/shNrf3 were seeded on 96-well plates at 5 × 10³ cells/well. Cells were treated with different concentrations of 5-FU. Cell viability was detected using CCK-8. (a, b) The inhibition rate was calculated after HT29 and HT29/Nrf3 were treated with different concentrations of 5-FU for 24 h and 48 h. These results showed that overexpressed Nrf3 increased the resistance of colon cancer cells to 5-FU. (c, d) The inhibition rate was calculated after SW620 and SW620/shNfr3 were treated with different concentrations of 5-FU for 24 h and 48 h. The results indicated that silencing Nrf3 increased the sensitivity of colon cancer cells to 5-FU. (e) The inhibition rate was calculated after HT29 and HT29/Nrf3 were treated with 5-FU (100 µg/ml) for different times (12 h, 24 h, 48 h). (f) The inhibition rate was calculated after SW620 and SW620/ShNrf3 were treated with 5-FU (50 µg/ml) for different times (12 h, 24 h, 48 h). (g) The effects of Nrf3 overexpression in HT29. (h) The silencing effects of Nrf3 in SW620.

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