Nrf3 Promotes 5-FU Resistance in Colorectal Cancer Cells via the NF-κB/BCL-2 Signaling Pathway In Vitro and In Vivo

Figure 4

Nrf3 blocked 5-FU-induced cell apoptosis. HT29, HT29/Nrf3, SW620, and SW620/shNrf3 were treated with different concentrations of 5-FU for 24 h. Cell apoptosis was determined by flow cytometry. (a) Representative images of cell apoptosis. (b, c) The proportion of apoptosis. Compared to HT29, the apoptosis rate of HT29/Nrf3 was decreased. The apoptosis rate of SW620/shNrf3 was increased compared with SW620. These results demonstrated that Nrf3 decreased the 5-FU-induced apoptosis rate. (d, e) The apoptosis-related proteins were detected by Western blot. The results showed that overexpressed Nrf3 reduced the expression of caspase-3, cleaved caspase-3, and caspase-9.

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