Ginsenoside Rh2 Inhibits Glycolysis through the STAT3/c-MYC Axis in Non-Small-Cell Lung Cancer

Figure 3

Rh2 inhibited NSCLC cell invasion and migration. (a) The invasion of A549 and H460 cells after treatment without or with 40 μg/ml Rh2 for 24 h was assessed using transwell assays. The invaded cells were stained, and the cell numbers were counted by ImageJ software. (b) The migration of A549 and H460 cells after treatment without or with 40 μg/ml Rh2 for 24 h was detected using wound healing assays. (c) The gap distance was measured and calculated by ImageJ software. Wound width (mm) = wound area/wound length. (d) After treatment with 40 μg/ml Rh2 for 48 h, the protein levels of the EMT markers E-cadherin, ZEB1, N-cadherin, and vimentin in NSCLC cells were determined by western blotting. (e) The relative western blot gray values are shown in the histogram. Data are shown as mean ± SD of three independent experiments. , vs. control groups.

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