Research Article
Ginsenoside Rh2 Inhibits Glycolysis through the STAT3/c-MYC Axis in Non-Small-Cell Lung Cancer
Figure 5
Rh2 induced metabolic shift via suppressing STAT3/c-Myc axis activity. (a) A549 and H460 cells were treated without or with 40 μg/ml Rh2 for 48 h. The protein levels of STAT3, p-STAT3 (Tyr 705), and c-Myc were tested by western blotting. (b) The relative western blot gray values are shown in the histogram. (c, d) Rh2 inhibited the nuclear translocation of p-STAT3 by western blot after nuclear and cytoplasm isolation. β-Actin and Histone H3 were used as cytoplasm and nuclear control, respectively. (e, f) Rh2 inhibited the nuclear translocation of p-STAT3 by immunofluorescence staining (10×). Scale bar = 100 μm. vs. control groups. ns, not significant.
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