Nucleolin Promotes Cisplatin Resistance in Cervical Cancer by the YB1-MDR1 Pathway

Figure 1

NCL is involved in cisplatin-resistant cervical cancer cells. (a) Western blot analysis and (b) quantification of NCL expression in cervical cancer, Hela cell, and cisplatin-resistant cervical cancer cell Hela/DDP. GAPDH was used as a loading control. , compared with Hela cells. (c) The NCL expression level was detected by western blot in Hela cells transfected with NCL overexpression vector or empty vector. (d) NCL overexpression cells were treated with graded concentrations of DDP; then, cellular viability was assessed by MTT assay. (e) The same as that in (c), but Hela/DDP cells were transfected with NCL siRNA or control siRNA. (f) Same as that in (d), but with NCL knockdown cells. The data in the curves represent mean ± SD from three independent experiments. (g, h) Edu proliferation assay analysis of the effect of NCL knockdown on the growth of Hela/DDP cells treated with DDP. , compared with control cells.

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