Nucleolin Promotes Cisplatin Resistance in Cervical Cancer by the YB1-MDR1 Pathway

Figure 5

NCL regulates the promoter activity of MDR1 in a YB1-dependent manner. (a) Structures of the MDR1 promoter/luciferase reporter plasmid and Y-Box mutated MDR1-luc plasmid. (b) Functional analysis of MDR1 promoter activity by luciferase assays: NCL overexpression plasmid or empty vector were cotransfected with the wildtype or mutant MDR1 promoter reporter vector into Hela cells and then treated with or without DDP. After that, the cells were lysed and the luciferase activity was measured. Data are mean ± SD, N = 3. (c) The same as that in (b), but the Hela/DDP cells were cotransfected with siRNA and MDR1 promoter/luciferase reporter plasmid.

(a)

(b)

(c)