RGS20 Promotes Tumor Progression through Modulating PI3K/AKT Signaling Activation in Penile Cancer

Figure 1

RGS20 is highly expressed in PC and associated with unfavorable progression-free survival. (a) The relative expression of RGSs in GSE57955 dataset (). The relative RGS expression was calculated with reference to normal penile tissue pool. (b) The RGS20 expression was not associated with HPV infection in GSE57955 dataset. HPV⁺ cases vs. HPV^- cases, . (c) Immunohistochemical staining showed the RGS20 expression in paired adjacent penile tissues or PC tissues, respectively. Bars: 50 μm. (d) Immunohistochemical staining showed that RGS20 was highly expressed in PC tissues compared to paired adjacent penile tissues (). (e) Immunohistochemical staining showed high or low RGS20 expression in PC tissues, bars: 50 μm. (f). Western blotting analysis on the expression of RGS20 in normal penile tissues (NPT1, NPT2) and a panel of PC cell lines (149RM, 149RCa, Penl1, and LM156). β-Actin was used as loading control. (g) Subcellular fractionation analysis showed that RGS20 was mostly present in the cytoplasmic fraction of PC cells. N: nuclear fraction ; C: cytoplasmic fraction; WCL: whole cell lysate.

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