RGS20 Promotes Tumor Progression through Modulating PI3K/AKT Signaling Activation in Penile Cancer

Figure 3

The overexpression of RGS20 enhances cell proliferation, soft agar clonogenesis, and migration/invasion in RGS20-low PC cell lines. (a) Western blotting on the RGS20 expression in 149RCa and LM156 cells transfected with empty vector (EV) or RGS20 lentiviruses. β-Actin was used as loading control. (b) CCK-8 analysis on cell viability following the RGS20 overexpression in PC cells. The cell viability in EV control was regards as 100%. , . (c) BrdU incorporation analysis on cell proliferation following the RGS20 overexpression in PC cells. The BrdU incorporation in EV control was regards as 100%. , . (d) Soft agar clonogenesis of PC cells following the RGS20 overexpression in PC cells. The soft agar clonogenesis in EV control was regards as 100%. , . Bars: 100 μm. (e) Caspase-3 activity of PC cells following the RGS20 overexpression. The caspase-3 activity in EV control was regards as 100%. , . (f) Wound healing assay on PC cells following the RGS20 overexpression. Bars: 100 μm. The cell migration in EV control was regards as 100%. , . (g) Transwell invasion assay on PC cells following the RGS20 overexpression. Bars: 50 μm. The cell invasion in EV control was regards as 100%. , .

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