RGS20 Promotes Tumor Progression through Modulating PI3K/AKT Signaling Activation in Penile Cancer

Figure 4

RGS20 regulates PI3K/AKT signaling activation in PC cells. (a) RNA-Seq analysis identified differentially expressed genes in Penl1 cells with or without RGS20 knockdown. (b) Top 5 enriched pathways for downregulated genes () following RGS20 knockdown in Penl1 cells. (c) GSEA analysis revealed that RGS20-high PC cases exhibited highly enriched PI3K/AKT signaling in GSE57955 dataset. (d) Coimmunoprecitation analysis on RGS20 interaction with PI3K p85α subunit in PC cells. IgG was used as coimmunoprecitation control. (e) PI3K activity of RGS20-depleted 149RM and Penl1 cells. The PI3K activity in Scr control was regards as 100%. , . (f) PI3K activity of 149RCa and LM156 cells with or without the RGS20 overexpression. The PI3K activity in EV control was regards as 100%. , . (g) PI3K activity of RGS20-depleted Penl1 cells after EGF stimulation. After serum deprivation for 36 hours, Penl1 cells (Scr or shRGS20) were stimulated with EGF (50 ng/mL) for 30 min, and then PI3K activity was analyzed. , . (h) Western blotting analysis on PI3K/AKT signaling proteins following RGS20 depletion in 149RM and Penl1 cells. (i) Western blotting analysis on PI3K/AKT signaling proteins following the overexpression of RGS20 in 149RCa and LM156 cells. (j) Western blotting analysis on PI3K/AKT signaling proteins in RGS20-depleted Penl1 cells after EGF stimulation. After serum deprivation for 36 hours, Penl1 cells (Scr or shRGS20) were stimulated with EGF (50 ng/mL) for 30 min. Western blotting was conducted to analyze PI3K/AKT signaling proteins.

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