RGS20 Promotes Tumor Progression through Modulating PI3K/AKT Signaling Activation in Penile Cancer

Figure 5

Knockdown of PI3K p85α or p110α suppresses cell proliferation, soft agar clonogenesis, and migration/invasion in PC cell lines. (a) Western blotting on PI3K p85α or p110α expression in 149RM and Penl1 cells transfected with scramble (Scr) control or shRNAs targeting p85α or p110α. β-Actin was used as loading control. (b) CCK-8 analysis on cell viability after p85α or p110α knockdown in 149RM and Penl1 cells. The cell viability in Scr control was regards as 100%. , . (c) BrdU incorporation analysis on cell proliferation following p85α or p110α knockdown in 149RM and Penl1 cells. The BrdU incorporation in Scr control was regards as 100%. , . (d) Soft agar clonogenesis of PC cells following p85α or p110α knockdown. The soft agar clonogenesis in Scr control was regards as 100%. , . Bars: 100 μm. (e) Caspase-3 activity of PC cells following p85α or p110α knockdown. The caspase-3 activity in Scr control was regards as 100%. , . (f) Wound healing assay on PC cells following p85α or p110α knockdown. Micrographs showed the results of Penl1 cells. Bars: 100 μm. The cell migration in Scr control was regards as 100%. , . (g) Transwell invasion assay on PC cells following p85α or p110α knockdown. Bars: 50 μm. The cell invasion in Scr control was regards as 100%. , .

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