Liensinine Inhibits Cell Growth and Blocks Autophagic Flux in Nonsmall-Cell Lung Cancer

Figure 1

Liensinine exerts cytotoxicity and induces apoptosis in vitro. (a) A549, H520, and SPC-A1 cells were treated with the indicated concentrations of liensinine for 24h and 48h, respectively, and then CCK-8 assay was performed. (b) A549, H520, and SPC-A1 cells were treated with the indicated concentrations of liensinine for 48h and then incubated with normal culture for 14 days. (c) A549, H520, and SPC-A1 cells were treated with indicated concentrations of liensinine for 48h, and apoptosis assays were performed using annexin V and PI staining. (d) A549 and SPC-A1 cells were treated with 20 μM liensinine with or without Z-VAD, and then apoptosis assay was performed. Three independent experiments were performed. The results were shown as means ± SD, .

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