Liensinine Inhibits Cell Growth and Blocks Autophagic Flux in Nonsmall-Cell Lung Cancer

Figure 3

Liensinine promotes mitochondrial dysfunction. (a) A549, H520, and SPC-A1 cells were treated with the indicated concentrations of liensinine for 48h and stained with COX IV antibody. Scale bars: 10 μm. (b–d) A549 and SPC-A1 cells were treated with liensinine or DMSO, and the mitochondria structure was observed by transmission electron microscope. The shape and area of mitochondria were measured and calculated. Scale bars: 0.4 μm. (e–f) A549, H520, and SPC-A1 cells were treated with the indicated concentrations of liensinine for 48h and then detected using JC-1 flow cytometry. Three independent experiments were performed. The results were shown as means ± SD, , , .

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