Liensinine Inhibits Cell Growth and Blocks Autophagic Flux in Nonsmall-Cell Lung Cancer

Figure 4

Liensinine induces autophagosome accumulation. (a) A549, H520, and SPC-A1 cells were treated with the indicated concentrations of liensinine for 48h, and Western blot assays demonstrated the LC3B expression. (b–c) LC3B puncta were observed by confocal laser microscopy after staining with LC3B antibody, and autophagic vacuoles were calculated. Scale bars: 10 μm. (d–e) A549 cells were treated with liensinine with or without Baf.A1 and then detected the LC3B puncta by immunofluorescence. LC3B puncta number per cell was quantified. (f) A549 cells were treated with liensinine with or without Baf.A1, and then Western blot assays demonstrated the LC3B expression. (g–h) A549 and H520 cells were treated with liensinine as indicated concentrations and then stained with SQSTM1 antibody. SQSTM1 puncta number per cell was quantified. Scale bars: 10 μm. Three independent experiments were performed. The results were shown as means ± SD, , .

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