Metformin Suppresses Hepatocellular Carcinoma through Regulating Alternative Splicing of LGR4

Figure 3

Metformin could reduce tumorigenesis through LGR4. (a). The expression of LGR4 was detected upon metformin treatment. (b). The proliferation of HCC by LGR4 overexpression in metformin-treated HCC cell lines. (c). The migration of HCC by LGR4 overexpression in metformin treated HCC cell lines; scar bar = 100 μm. (d). The invasion of HCC by LGR4 overexpression in metformin-treated HCC cell lines; scar bar = 50 μm. (e). The expression of catenin by LGR4 overexpression overexpressed metformin-treated HCC cell lines. ^∗∗, ^∗∗∗. PcDNA, an empty vector.

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