Metformin Suppresses Hepatocellular Carcinoma through Regulating Alternative Splicing of LGR4

Figure 4

Metformin decreased the LGR4 expression by alternative splicing. (a). Volcano plots shows genes with differential transcript usage (differential isoform fraction (dIF) > 0.3 and FDR < 0.01). (b). KEGG pathways enriched for genes with differential transcript usage. (c). Visualization of switched isoform structure LGR4. Three isoforms showed differential isoform expressions, although there was no difference for the overall gene expression. Treated: metformin treatment.

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