Metformin Suppresses Hepatocellular Carcinoma through Regulating Alternative Splicing of LGR4

Figure 5

LGR4 switching genes RNAi reduces the antitumor effect caused by metformin. (a). The proliferation of HCC by LGR4 switching genes RNAi in metformin-treated HCC cell lines. (b). The migration of HCC by LGR4 switching genes RNAi in metformin-treated HCC cell lines; scar bar = 100 μm. (c). The invasion of HCC by LGR4 switching genes RNAi in metformin-treated HCC cell lines; scar bar = 50 μm. (d). The expression of catenin was induced by LGR4 switching genes by RNAi metformin-treated HCC cell lines. ^∗∗, ^∗∗∗. PcDNA, an empty vector.

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