The lncRNA NEAT1/miRNA-766-5p/E2F3 Regulatory Axis Promotes Prostate Cancer Progression

Figure 4

miRNA-766-5p targeted 3′-UTR of NEAT1. (A) The location of NEAT1. , nuclear vs. cytoplasmic. (b) The binding sites between NEAT1 and miRNA-766-5p were predicted by StarBase. (c) The relationship between NEAT1 and miRNA-766-5p was testified by the luciferase reporter assay. , miR-766-5p mimic vs. NC mimic. (d) The level of miRNA-766-5p in PCa tissues. , tumor vs. nontumor. (e) The level of miRNA-766-5p in PCa cell lines. , P4E6, LNCaP, PC3, and DU145 vs. RWPE-1. (f) The interaction between NEAT1 and miRNA-766-5p was testified by the RIP assay. , Ago2 vs. IgG. (g) The level of miRNA-766-5p in sh-NEAT1-treated cells. , sh-NEAT1 vs. sh-NC. (h) Pearson’s correlation analysis demonstrated the correlation of NEAT1 expression with miRNA-766-5p.

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