CircNRIP1 Exerts Oncogenic Functions in Papillary Thyroid Carcinoma by Sponging miR-653-5p and Regulating PBX3 Expression

Figure 1

circNRIP1 is increased in PTC and circNRIP1 knockdown attenuates the malignant progression of PTC. (a) circNRIP1 expression in PTC tissues was determined by qRT-PCR assay. (b) circNRIP1 expression in PTC tissues with or without distant metastasis was detected using qRT-PCR assay. (c) circNRIP1 expression in PTC tissues with different TNM stages was evaluated with qRT-PCR assay. (d) The relationship between circNRIP1 expression and overall survival was estimated using Kaplan-Meier analysis. (e) circNRIP1 expression in PTC cell lines was determined by qRT-PCR assay. (f) The success of knockdown of circNRIP1 with si-circ-#1/2 in PTC cells was confirmed by qRT-PCR analysis. (g–i) CCK-8 and EdU incorporation assay were used to detect the cell proliferation. (j) Tunel staining was used to evaluate the apoptosis of tumor cells. (k) Western blot was conducted to evaluate the apoptosis biomarkers. (l, m) The cell migration and invasion abilities were detected with transwell assay. .

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