CircNRIP1 Exerts Oncogenic Functions in Papillary Thyroid Carcinoma by Sponging miR-653-5p and Regulating PBX3 Expression

Figure 2

circNRIP1 directly binds to miR-653-5p. (a) The location of circNRIP1 was verified by the subcellular fractionation assay. (b, c) The potential binding microRNA expressions after knockdown of circNRIP1 were determined by qRT-PCR analysis. (d, e) The miR-653-5p expressions in PTC tissues and cell lines were determined by qRT-PCR assay. (f) The binding sites of miR-653-5p in circNRIP1. (g, h) Luciferase reporter assay and RNA pull down assay were carried out to investigate the physical connection between circNRIP1 and miR-653-5p. (i) The success of miR-653-5p inhibition with miR-653-5p inhibitor in PTC cells was confirmed by qRT-PCR analysis. (j) EdU incorporation assay was used to detect the cell proliferation. (k) Tunel staining was used to evaluate the apoptosis of tumor cells. (l, m) The cell migration and invasion abilities were detected with transwell assay. .

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