CircNRIP1 Exerts Oncogenic Functions in Papillary Thyroid Carcinoma by Sponging miR-653-5p and Regulating PBX3 Expression

Figure 3

PBX3 is a direct target of miR-653-5p. (a) The binding site between miR-653-5p and the 3-UTR of PBX3. (b) The PBX3 expression in PTC cell lines was determined by qRT-PCR assay. (c) Luciferase reporter assay was conducted to investigate the connection between miR-653-5p and PBX3 in HEK-293T. (d) RIP assay was carried out to determine the relationship among miR-653-5p, circNRIP1, and PBX3. (e) The success of PBX3 inhibition with sh-PBX3 in PTC cells was confirmed by qRT-PCR analysis. (f) EdU incorporation assay was used to detect the cell proliferation. (g) Tunel staining was used to evaluate the apoptosis of tumor cells. (h, i) The cell migration and invasion abilities were detected with transwell assay. .

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