DNAJA1 Stabilizes EF1A1 to Promote Cell Proliferation and Metastasis of Liver Cancer Mediated by miR-205-5p

Figure 1

DNAJA1 promoted proliferation of liver cancer cells in vitro. (a) The mRNA level of DNAJA1 in six liver cancer cell lines detected by q-PCR. (b) The protein expression of DNAJA1 in six liver cancer cell lines detected by western blot. Error bars represent from three independent experiments. . (c) Relative protein expressions of DNAJA1 in Huh 7 and HepG2 cells stably transfected with DNAJA1 overexpression vector or control vector by western blot. (d) Relative protein expressions of DNAJA1 in MHCC-97H and HCC-M3 cells stably transfected with DNAJA1 shRNA or negative control by western blot. (e and f) Effect of DNAJA1 overexpression or downregulation on cell proliferation of Huh 7 and HepG2 cells or MHCC-97H and HCC-M3 cells detected by CCK-8 assay. (g and h) Effect of DNAJA1 overexpression or inhibition on cell clone formation ability of HepG2 cells or HCC-M3 cells.

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