Hsa-let-7d-5p Promotes Gastric Cancer Progression by Targeting PRDM5

Figure 1

Let-7d-5p is overexpressed in GC cells, and knockdown of let-7d-5p inhibits proliferation of GC cells. AGS and SGC-7901 cells were transfected with a let-7d-5p inhibitor. (a) qRT-PCR tested the expression of let-7d-5p in GC cell lines (AGS and SGC-7901) and GSE-1 cells. Values are mean ± SD vs. GSE-1. n = 3 per group. (b) qRT-PCR analysis to detect the knockdown efficiency of let-7d-5p. (c) CCK-8 assays on the cell proliferation at the indicated time points in AGS and SGC-7901 cells (24, 48, and 72 h). (d) Colony formation assays in AGS and SGC-7901 cells. (e) Flow cytometry analyzed the apoptosis of cells. (f) The expression levels of antiapoptotic protein (Bcl-2) and proapoptosis proteins (Bax, cleaved caspase-3, and cleaved caspase-9) were detected by Western blot. Values are mean ± SD, , vs. NC inhibitor, n = 3 per group.

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