Dual Roles of Lactate in EGFR-TKI-Resistant Lung Cancer by Targeting GPR81 and MCT1

Figure 2

Lactate enhances AKT expression by targeting GPR81-promoting TKI resistance. (a) GPR81 expression was evaluated on HCC827 and PC9 cells by flow cytometry with (blue) or without (red) 10 mM lactate pretreatment. (b) AKT expression of HCC827 and PC9 cells was measured with western blot with or without 10 mM lactate pretreatment. (c) HCC827 cells were cultured with siRNA GPR81 or control (siCT), and then treated with 10 mM lactate. AKT expression was analyzed with immunofluorescence assays (stained red). (d) HCC827 cells were cultured with siRNA GPR81 or control (siCT), and then treated with 10 mM lactate and 2 μM erlotinib. The percentage of apoptosis was measured with flow cytometry. All values are mean ± S.D. (n ≥ 3). . Bars represent 20 μm.

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