Dual Roles of Lactate in EGFR-TKI-Resistant Lung Cancer by Targeting GPR81 and MCT1

Figure 3

MCT1 increases lactate influx enhancing glycolysis and cell viability. (a) MCT1 expression was evaluated on HCC827 and PC9 cells by flow cytometry with (blue) or without (red) 10 mM lactate pretreatment. (b) Extracellular acidification rate (ECAR) was used to measure glycolysis efficacy in HCC827 cells treated with 10 mM lactate, 10 mM lactate combined with AZD3965 or control. (c) Cell viability was measured in the condition of 10 mM lactate, 10 mM lactate combined with AZD3965 or control. Data are present as mean ± SD. (n ≥ 3). .

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