Hsa_circ_0000520 Promotes Non-Small Cell Lung Cancer Progression through the miR-1258/AKT3 Axis

Figure 1

Circ_0000520 is upregulated in NSCLC tissues and cells. (a) The volcano plot displays the expression differences of circRNAs in the data set GSE158695 (cutoff criterion: log₂|fold change| > 1 and ). The downregulated circRNAs are marked in blue, and the upregulated circRNAs are marked in red. Black represents circRNAs with no significant difference. (b) Detection of circ_0000520 expression in NSCLC tissues and para-cancerous tissues by qPCR. (c) Detection via qPCR of circ_0000520 expression in NSCLC cell lines (A549, H460, NCI-H2106, NCI-H1299, and H1975) and human immortalized bronchial epithelial cells (BEAS-2B). (d) Nucleocytoplasmic separation experiment was performed to detect the subcellular localization of circ_0000520 in NSCLC cells. AuthorAnonymous. (e) Kaplan–Meier survival analysis was conducted to examine the correlation between the circ_0000520 expression and the overall survival rate of NSCLC patients. and .

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