Hsa_circ_0000520 Promotes Non-Small Cell Lung Cancer Progression through the miR-1258/AKT3 Axis

Figure 5

Circ_0000520 plays a part by regulating the miR-1258/AKT3 axis. (a) The binding site between miR-1258 and AKT3 3′-UTR was predicted by the TargetScan database. (b) Dual-luciferase reporter gene assay was utilized to verify the binding relationship between miR-1258 and AKT3 3′-UTR. (c, d) Western blot was conducted to detect the level of AKT3 protein in A549 and H460 cells transfected with pcDNA3.1-NC, pcDNA3.1-circ_0000520, and pcDNA3.1-circ_0000520+miR-1258 mimics. (e) Detection by qPCR of the AKT3 mRNA expression in NSCLC tissues and para-cancerous tissues. (f, g) Pearson’s correlation analysis of the correlation between the AKT3 mRNA expression and miR-1258 or circ_0000520 expression in NSCLC tissues. (h) qPCR was utilized to detect AKT3 mRNA expression in NSCLC cell lines and BEAS-2B cells. and .

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